Counting Chromosomes in a Nucleus

Capturing clear images without fluorescence blurring

FISH (Fluorescence In Situ Hybridization) is a method of detecting the distribution or amount of specific nucleic acids (DNA or RNA) using fluorochromes. It is mainly used for detecting chromosomal abnormalities.

Normal observation

Normal observation

Sectioning + Z-stack

Sectioning + Z-stack

Hybrid Cell Count

Hybrid Cell Count

Objective lens: CFI Plan Apo λ 100xH
Sectioning + Z-stack

Using the All-in-One Fluorescence Microscope BZ-X800

  • The Sectioning function makes it possible to eliminate fluorescence blurring optically and capture clear images.
  • A built-in Z-stack function captures multiple images at different focal positions and is able to create a fully focused image by combining only the areas that are at their sharpest focus.
  • Hybrid Cell Count can be used to specify nuclei as mask areas to extract and count chromosomes contained in each nucleus.

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No. of nuclei containing respective signals

Signal
G\R
0 1 2 3 4

40

5

1

1

0

23

35

13

1

0

10

24

18

2

2

0

5

7

2

0

1

3

4

2

1